anti prostaglandin endoperoxide synthase 2 (Proteintech)

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Proteintech anti prostaglandin endoperoxide synthase 2

PRDX4 is an important regulator of ferroptosis in ESCC cells. (A) Determination of MDA, LPO and GSH contents in KYSE270 cells after transfection with PRDX4 siRNA. (B) Western blot analysis of the protein levels of GPX4, SLC7A11 and <t>PTGS2</t> in KYSE270 cells transfected with PRDX4 siRNA. (C) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE270 cells transfected with PRDX4 siRNA. (D) Determination of MDA, LPO and GSH contents in KYSE30 cells after transfection with pcDNA3.1-PRDX4. (E) Western blot analysis of the protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (F) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (G) Detection of the levels of MDA, LPO and GSH in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (H) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (I) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (J) Detection of the levels of MDA, LPO and GSH in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (K) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (L) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. ** P<0.01, *** P<0.001 and **** P<0.0001, indicate statistical significance. PRDX4, peroxiredoxin 4; ESCC, esophageal squamous cell carcinoma; siRNA, small interfering RNA; MDA, malondialdehyde; LPO, lipid peroxidation; GSH, glutathione; GPX4, glutathione peroxidase 4; SLC7A11, solute carrier family 7 member 11; PTGS2, prostaglandin-endoperoxide synthase 2; Fer-1, ferrostatin-1; ns, not significant.

Anti Prostaglandin Endoperoxide Synthase 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1089 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cox2/COX2%2F+Cyclooxygenase+2%2F+PTGS2+Antibody/pmc13040322-90-57-66
Average 96 stars, based on 1089 article reviews

anti prostaglandin endoperoxide synthase 2 - by Bioz Stars, 2026-10

96/100 stars

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1) Product Images from "Peroxiredoxin 4 suppresses ferroptosis in esophageal squamous cell carcinoma by activating the phosphoinositide 3-kinase signaling pathway"

Article Title: Peroxiredoxin 4 suppresses ferroptosis in esophageal squamous cell carcinoma by activating the phosphoinositide 3-kinase signaling pathway

Journal: Biomedical Reports

doi: 10.3892/br.2026.2133

Figure Legend Snippet: PRDX4 is an important regulator of ferroptosis in ESCC cells. (A) Determination of MDA, LPO and GSH contents in KYSE270 cells after transfection with PRDX4 siRNA. (B) Western blot analysis of the protein levels of GPX4, SLC7A11 and PTGS2 in KYSE270 cells transfected with PRDX4 siRNA. (C) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE270 cells transfected with PRDX4 siRNA. (D) Determination of MDA, LPO and GSH contents in KYSE30 cells after transfection with pcDNA3.1-PRDX4. (E) Western blot analysis of the protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (F) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (G) Detection of the levels of MDA, LPO and GSH in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (H) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (I) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (J) Detection of the levels of MDA, LPO and GSH in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (K) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (L) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. ** P<0.01, *** P<0.001 and **** P<0.0001, indicate statistical significance. PRDX4, peroxiredoxin 4; ESCC, esophageal squamous cell carcinoma; siRNA, small interfering RNA; MDA, malondialdehyde; LPO, lipid peroxidation; GSH, glutathione; GPX4, glutathione peroxidase 4; SLC7A11, solute carrier family 7 member 11; PTGS2, prostaglandin-endoperoxide synthase 2; Fer-1, ferrostatin-1; ns, not significant.

Techniques Used: Transfection, Western Blot, Control, Expressing, Small Interfering RNA

Western Blot:

Article Title: Peroxiredoxin 4 Involved in Spermatogenesis by Affecting Oxidative Stress and Ferroptosis.
Article Snippet: .. Proteins were resolved by SDS-PAGE, ransferred to polyvinylidene difluoride (PVDF) membranes and hen analyzed by immunoblot using primary antibodies includng anti-ACSL4 (1:1000, RM8098, Biodragon, Suzhou, China), nti-ATF6 (1:2000, 24169-1-AP, Proteintech, Illinois, USA), anti-actin (1:1000, 66009-1-Ig, Proteintech), anti-Bax (1:1000, 50599- -Ig, Proteintech), anti-Beclin (1:1000, 11306-1-AP, Selleck, Texas, SA), anti-BIP (1:500, 66574-1-Ig, Proteintech), anti-CAT (1:2000, AB1405562, Sigma–Aldrich, Missouri, USA), anti-COX2 (1:1000, M6051, Biodragon), anti-GAPDH (1:1000, 60004-1-Ig, Proteinech), anti-GPX4 (1:1000, 52455S, CST, Massachusetts, USA), anti53 (1:1000, 10442-1-AP, Proteintech), anti-p62 (1:1000, E1462, Seleck), anti-SLC7A11 (1:1000, 26864-1-AP, CST), anti-SOD1 (1:1000, b308181, Abcam), anti-StAR (1:1000, 67130-1-Ig, Proteintech), ndrology, 2026 anti-Vinculin (1:1000, 13901S, CST), and anti-XBP1S (1:1000, 24868-1-AP, Proteintech) antibodies. ..

Incubation:

Article Title: Sequential delivery of sinigrin and dabigatran by an in situ self-stabilizing dynamic hydrogel attenuates intervertebral disc degeneration
Article Snippet: .. The membranes were then incubated overnight with anti-GAPDH (1:50 000, AC054, abclone, China), anti-Col2α1(1:500, A19308, abclone, China), anti-Acan (1:1000, A8536, abclone, China), anti-SOX9 (1:1000, A19710, abclone, China), anti-MMP13 (1:1000, A1606, abclone, China), anti-ADAMTS4 (1:500, A2525, abclone, China), anti-iNOS (1:1000, 18985-1-AP, proteintech, China), anti-COX2 (1:1000, 12375-1-AP, proteintech, China), anti-ERK (1:2000, 11257-1-AP, proteintech, China), anti-Phospho-ERK (1:1000, 28733-1-AP, proteintech, China), anti-JNK (1:500, 51153-1-AP, proteintech, China), anti-Phospho-JNK (1:1000, 80024-1-RR, proteintech, China), anti-p38 (1:1000, A4771, abclone, China), anti-Phospho-p38 (1:500, AP1508, abclone, China), anti-p65 (1:5000, A19653, abclone, China), anti-Phospho-p65 (1:500, AP0475, abclone, China), anti-IκBα (1:500, A1187, abclone, China), anti-Phospho-IκBα (1:500, AP0420, abclone, China), anti-AMPK (1:5000, A28094, abclone, China), anti-Phospho-AMPK (1:500, AP0432, abclone, China), The membrane was incubated with HRP-conjugated Goat anti-rabbit IgG (H + L) (1:50 000, AS014, rabbit monoclonal, Abclone, China) and immunoreactive proteins were detected using enhanced chemiluminescence. .. Images were captured using the Bio-Rad ChemiDoc Touch Imaging System, and protein band density was analyzed using ImageJ software.

Article Title: Dexmedetomidine alleviates sepsis-associated acute kidney injury by inhibiting renal tubular ferroptosis via JNK/MAPK–LCN2 pathway
Article Snippet: Equal amounts of protein were separated by 10% Tris–Glycine extended stain-free SDS-PAGE gels (Bio-Rad, USA) and transferred onto polyvinylidene difluoride (PVDF) membranes (Roche, USA) using a wet transfer apparatus (Bio-Rad, USA). .. Membranes were blocked with 5% non-fat milk at room temperature for 1.5 h, followed by overnight incubation at 4 °C with the following primary antibodies: Anti-LCN2 (Proteintech, 26991–1-AP, 1:1000) for HK-2 cells, Anti-LCN2 (Abcam, ab216462, 1:1000) for murine renal tissues, Anti-ACSL4 (Abcam, ab155282, 1:20000), Anti-COX2 (Proteintech, 66351–1-Ig, 1:2000), Anti-xCT (Abcam, ab175186, 1:4000), Anti-GPX4 (CST, #52455, 1:1000), Anti-JNK (CST, #9252, 1:1000), Anti-p-JNK (CST, #4671, 1:1000), Anti-ERK (Proteintech, 11257–1-AP, 1:5000), Anti-p-ERK (Proteintech, 28733–1-AP, 1:3000), Anti-p38 (Proteintech, 14064–1-AP, 1:1000), Anti-p-p38 (Proteintech, 28796–1-AP, 1:2000). .. After washing, membranes were incubated with secondary antibodies at room temperature for 1.5 h: Goat anti-rabbit IgG (CST, #7074, 1:3000), Goat anti-mouse IgG (CST, #7076, 1:3000).

Membrane:

Marker:

Article Title: Targeting Microglial CD49a Inhibits Neuroinflammation and Demonstrates Therapeutic Potential for Parkinson's Disease.
Article Snippet: An anti-IBA1 primary antibody (ab178846) was acquired from Abcam plc (Cambridge, UK). .. Anti-iNOS (18985-1-AP), anti-COX2 (12375-1-AP), anti-PGAM5 (28445-1-AP), anti-NLRP3 (30109-1-AP), and anti-ASC (83858-3-RR) primary antibodies were purchased from Proteintech (Wuhan, China). anti-Actin (AA128) primary antibody, horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H + L) (A0208), HRP-labeled goat anti-mouse IgG (H + L) (A0216) secondary antibodies, and prestained color protein marker (P0068; P0071) were acquired from Beyotime Biotechnology (Shanghai, China). .. Goat Antimouse IgG (H + L) (DyLight 647 conjugate) (BA1151), Goat anti-mouse IgG (H + L) (DyLight 550 conjugate) (BA1133), and Goat anti-rabbit IgG (H + L) (DyLight 550 conjugate) (BA1135) secondary antibodies were obtained from Boster Biotechnology (Wuhan, China).

Article Title: Targeting Microglial CD49a Inhibits Neuroinflammation and Demonstrates Therapeutic Potential for Parkinson's Disease
Article Snippet: An anti‐IBA1 primary antibody (ab178846) was acquired from Abcam plc (Cambridge, UK). .. Anti‐iNOS (18985‐1‐AP), anti‐COX2 (12375‐1‐AP), anti‐PGAM5 (28445‐1‐AP), anti‐NLRP3 (30109‐1‐AP), and anti‐ASC (83858‐3‐RR) primary antibodies were purchased from Proteintech (Wuhan, China). anti‐Actin (AA128) primary antibody, horseradish peroxidase (HRP)‐labeled goat anti‐rabbit IgG (H+L) (A0208), HRP‐labeled goat anti‐mouse IgG (H+L) (A0216) secondary antibodies, and prestained color protein marker (P0068; P0071) were acquired from Beyotime Biotechnology (Shanghai, China). .. Goat Anti‐mouse IgG (H+L) (DyLight 647 conjugate) (BA1151), Goat anti‐mouse IgG (H+L) (DyLight 550 conjugate) (BA1133), and Goat anti‐rabbit IgG (H+L) (DyLight 550 conjugate) (BA1135) secondary antibodies were obtained from Boster Biotechnology (Wuhan, China).

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